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LIQUID LIQUID CHROMATOGRAPHY CAN GREATLY ENHANCE BOTH YOUR RESEARCH AND PROCESS PRODUCTION
COUNTER CURRENT & CENTRIFUGAL PARTITION CHROMATOGRAPHY, PLUS HPLC, IL-LC AND SFC
Together AECS-QuikPrep (UK) and Couturier (France) bring you World Leading products for
High Performance Liquid-Liquid Chromatography™ at scales from micro-gram to tonnes per annum
High Performance Liquid-Liquid Chromatography™ at scales from micro-gram to tonnes per annum
Introduction, History, Principles & Advantages of CCC; Specifications, Coil Options, Modular
Upgrades, Safety & Reliability Matters, Additional Safety Options, Introducing Biphasic Solvent
Choices, Key Applications, Some Quattro CCC™ Users
Your 1st Choice for Preparative and Process Chromatography / Extraction
Introducing application of Quattro CCC™ use in NEEM Research, Structures of 7 highly complex
tetranortriterpenoids in NEEM sought as targets, Existing methodology (very time consuming
with poor recovery), More proof biphasic methods for one CCC design often not compatible
different CCC design, (NOTE IMPLICATIONS TO MANUFACTURERS WHO USE DIFFERENT
DESIGNS IN THEIR PRODUCT RANGE)(Why all QUATTRO CCC share all key CCC design
parameters)(Believe competitor's propaganda at potentially your own considerable
risk and cost), Quattro CCC Success using Sequential CCC & HPLC, Our new biphasic solvent
system choice. Results, Approximately 100% MASS BALANCE, injected material to CCC
recovery, Summary 7 pure targets achieved at 96+% purity and 95+% recovery.
Introducing application Quattro CCC™ use in Wine Research, Preliminary choice of biphasic
solvent, Structures of complex, native, anthocyanins, Optimisation of biphasic solvent system
and instrument parameters (NOTE RESULTS SUGGESTED IT WAS PREFERABLE TO REDUCE
ROTATION SPEED FOR THIS BIPHASIC SYSTEM/APPLICATION)(Again we caution that
you do not believe competitor's propaganda that HIGH "G" / Very High Speeds are
always better, we also refer you to excellent text book by Dr Walter Conway, that
also contests the High "G" ; high speed mantra, giving solvent systems with
different speeds for optimal stationary phase retention, many of which become
sub-optimal at too high a speed for certain biphasic solvent systems)(Note,
University publishing this false High "G" ; high speed mantra mantra, also
co-founders and shareholders in competitor pushing this false High "G" / very high
speed mantra)(oops), Use step gradient and elution/extrusion, Results with online
chromatographs plus off-line HPLC-MS of fractions, Industrial scale considerations, Summary
Important examples of isocratic, linear gradient, plus step gradient use utilising
Quattro CCC™, (Note: In addition to slides, additional explanatory comments are available with
this slide show), Applications, Polyphenols in natural products, Laboratory Scale-Up, Engine Oil
Anti-Wear Agents, Polarity window concept for isocratic use with selected targets, Utilising
same targets to show gradient application to extend polarity window. Comparison Quattro
CCC™ to Flash Chromatography, Utilising gradient HPLC to predict generic gradient CCC
biphasic strategies, Gradient applications to natural products, Further discussion linear as
compared step gradients, Discussion normal and recerse phase gradients on same samples,
Example gradient achieve high purity and pre-concentrating target in complex natural product,
GENERIC ENGINE OIL TOTAL POLARITY SCREEN FROM 200 TO 500,000 MOLECULAR
WEIGHT UTLISING GRADIENT AND AECS MOVING BELT HPLC-FID DETECTOR, AECS MOVING
BELT HPLC-FID / HPLC-MS , CCC-MS DETECTOR
Application for bioactive phenylpropanoid & iridoid glycosides, Pictures early Craig & PC Inc
L-LC, Gradient elution, Optimising biphasic solvent, Optimising step gradient, Results, Summary
Application for bioactive pyridine derivatives, Open tubular silica failed, Choice of CCC, Why
gradient elution, Optimising biphasic solvent, Optimising step gradient utilising one of AECS's
generic strategies, Additional TLC testing for partition coefficient determination, Results,
Summary
A discussion comparing AECS's Flash Chromatography, HPLC and L-LC products, when should
you use which alternative? Choice of chromatography methodology, Flash Chromatography,
Laboratory and Process Preparative Flash Chromatography, AECS CUSTOM DESIGNS HPLC
PHASES (note often we design HPLC phases etc for third parties, who then manufacture
themselves, sometimes we still do synthesis, and allow others to OEM retail these unique HPLC
phases), L-LC applications, AECS Moving Belt CCC/HPLC-FID Interface, also interfaces to Micro
Wave Plasma and MS Detectors. (Note: AECS also custom designs instrumentation for a variety
of Instrumentation Companies).
Click on the slides below to view or download PDF