COUNTER CURRENT & CENTRIFUGAL PARTITION CHROMATOGRAPHY, PLUS HPLC, IL-LC AND SFC
Together AECS-QuikPrep (UK) and Couturier (France) bring you World Leading products for High Performance Liquid-Liquid Chromatography™ at scales from micro-gram to tonnes per annum
• CCC can cope with a wide range of radically different polarity compounds.
• CCC can cope with particulates and extract solid samples.
• CCC can have orthogonal selectivity to HPLC.
• CCC can achieve 99 + % purity of target compounds from complex samples.
• CCC can take extremely complex matrices, such as natural product extracts and heart cut
target polarities, bio-actives etc without risk of on-column degradation or adsorption. The
achieved narrow cut of polarity will be of such a small polarity range, that it is ideal for
injection into a 5um preparative 21 x 250 mm Preparative C18 HPLC column to resolve final
target, if required purity not originally achieved by CCC
• CCC uses exactly the same type of liquid pumps, injectors, switching valves fraction
collectors etc as HPLC, flash chromatography or SMB, but as CCC often requires only a
fraction of the solvent flow or total solvent used, CCC offers substantial cost savings in
capital equipment cost by using lower flow pumps, plus savings in solvent and process cost.
Standard analytical 10 ml/min HPLC systems can be utilised on 1 litre + Quattro CCC™ for 5
to 40 g "on-column injections. Why not compare that to competitors needs in HPCCC or
Preparative Flash Chromatography or Preparative HPLC?
COUNTER CURRENT CHROMATOGRAPHY IS COMPLIMENTARY TO PREPARATIVE HPLC
Strengths of Counter Current Chromatography for chromatography aspects
Your 1st Choice for Preparative and Process Chromatography / Extraction
Quattro CCC/HPL-C Compliments HPLC
• Scale up is usually predictable for the modular Quattro CCC™.
• Small laboratory 100 ml coil CCC can rapidly process 0.5 to 4 + grams of crude extract, but
only require flows of 2 to 10 ml /min and therefore can use a standard analytical HPLC pump.
• Single process units of 3000 ml can rapidly prepare 15 to 120 + grams of crude extract, but
only require flows of 25 to 100 ml /min. HPLC Pumps of this capacity are available for modest
• Any number of process units can be used in series / manifolded in parallel / or used in
Simulated Moving Bed (SMB) mode for tonne per annum production.
• CCC solvent selection choice is unfamiliar to many chromatographers.
• CCC fundamental principles are unfamiliar to many chromatographers.
• Choice of phase and solvent optimisation familiar to most chromatographers.
• Traditional preparative/process chromatographers understand scale up.
Weaknesses of Preparative HPLC
The individual costs of high-resolution 5 or 10um particle size columns of 250mm length by 1
or 2 inch diameter are relatively high; yet the sample loading may be only 0.5 to 2 grams.
The typical eluent flows would be 10 to 50 + ml / min and these flows would require a
dedicated Preparative HPLC pump.
Columns can be easily and permanently incapacitated by injection of crude extract containing
a wide mix of polarities.
Equipment to pack columns of larger than 2 inch bore requires a large one-off cost, plus
requires the purchase of HPLC phase as a consumable.
Columns capable of loadings of 15 to 120 grams would require flows of 100's ml /min to
1000 + ml /min, and therefore require specialist and very expensive HPLC pumps plus solvent
Scale up from 250 x 4.6 mm id analytical columns with 3 to 5um particle to larger
preparative columns often with 7, 10, 15 or 20 um particle sizes is not familiar to many
analytical chromatographers. This is especially so as most analytical chemists use aqueous
reverse phase C18 chromatography. But because aqueous solvents are difficult to evaporate,
most large-scale chromatographers preferentially utilise organic normal phase
chromatography. This is unfamiliar to many analytical chromatographers.
Complimentary Nature of Counter Current Chromatography to Preparative HPLC
When suitable biphasic solvent systems are chosen a Quattro CCC™ can prepare a highly
pure ( 99 + % ) target compound, directly from a complex matrix.
Sometimes, however, the time and or method optimisation familiarity is not available to
find a successful CCC method in an allotted time scale.
AECS Generic Counter Current Chromatography step gradient techniques are available to
heart cut target polarities from crude, complex mixtures. This CCC technique can both
radically reduce the mass needing to be prepared by the preparative HPLC, plus
substantially reduce the likelihood of contaminating the HPLC column.
As an example.......consider a crude natural product extract of 50 grams with 1% active.
This could not be considered directly by preparative HPLC, but would only require a 3000
ml Quattro CCC™ running at 50 ml /min to heart cut approximately 1 grams of a narrow
polarity band. A 250 mm long x 1 inch diameter preparative HPLC column with 10um HPLC
reverse phase running at 25 ml /min using standard HPLC solvent and phase optimisation
techniques, could then rapidly prepare the target, without risk of irreversible column
Now scale this down from 50 grams of sample to 1 grams of sample.
A small 100 ml Quattro CCC™ coil running at modest flows available from standard
analytical HPLC pumps, could rapidly prepare 10mg of a narrow polarity extract.
Consider the cost savings of a 10 mg Preparative HPLC column plus ancillary equipment,
compared to a 1 gram column with the appropriate ancillary equipment, and factor in the
lack of risk of column contamination after Counter Current Chromatography heart cutting.
Worth Thinking About ? Require More Information
Strengths of Counter Current Chromatography for scale-up aspects
Quattro HPL-LC™ / HTPrep™ Video (2 bobbins, 4 coils / 3 coil id’s). Door safety
mechanism disabled for this demonstration video.Set spin speeds are very constant. The video
judder is caused by camera/internet